mAb anti-Amyloid β Peptide 42, CA9 3F8

Description

Description: Mouse monoclonal antibody to C-terminal of amyloid beta peptide 42 (Aβ42) 

Purification: Protein G affinity purified

Product Type: Primary antibody

Target Protein: C-terminal of amyloid beta peptide 42

Immunogen: KLH conjugated to a short peptide (MVGGVVIA) with amino acid sequence corresponding to the C-terminal of Aβ42

Fusion Myeloma: Sp2/0-Ag14

Specificity: This antibody recognizes the C-terminal sequence (MVGGVVIA) of Aβ42 and full length Aβ42.

Cross-Reactivity: The antibody showed very weak to none cross-reactivity with full length Aβ43 and Aβ40 in indirect ELISA. 

Species Reactivity: Human and other primates; mouse, rat

Host / Isotype: Mouse, IgG2b Kappa

Formulation: Lyophilized from a solution in 0.01M PBS pH7.2

Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20^oC

Research Area: Aging and neurodegenerative diseases

Background: 

Amyloid beta peptide 42 (Aβ42) is best known for its role in the formation of senile plaques in the brain of patients with Alzheimer’s disease.  Aβ42 and Aβ40 are the two major amyloid peptides that are produced after cleavage of amyloid precursor protein by secretases.   Aβ42 (42 amino acids) is very fibrillogenic.  The beta pleated structure of Aβ42 constituents the initial and key component of the insoluble amyloid fibril in senile plaque.  It is widely accepted that Aβ42 contributes to the pathogenesis of Alzheimer’s disease.  One proposition is that the deposition of amyloid fibril onto the brain tissue results in Alzheimer’s disease.  Another is that the neurotoxicity of Aβ42 oligomer is the cause of the disease.

Application: 

IHC: The picture below shows the 40x amplified brain tissue section from transgenic Alzheimer’s disease mouse model after IHC staining with clone CA9 3F8.

(Procedure:  After heat induced epitope retrieval, formalin-fixed paraffin-embedded brain tissue section from 12 month old APPswe/PSEN1dE9 mouse was incubated at 4^oC with 0.01mg/ml CA9 3F8 overnight.  Bound antibody was detected using a HRP-labeled goat anti-mouse antibody.)

References:

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